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    GenScript corporation gastv orf2
    Schematic diagram of recombinant plasmid <t>pEP-BGH-GAstV</t> <t>ORF2</t>
    Gastv Orf2, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gastv orf2/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    gastv orf2 - by Bioz Stars, 2026-06
    90/100 stars

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    1) Product Images from "In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector"

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    Journal: BMC Veterinary Research

    doi: 10.1186/s12917-025-04654-7

    Schematic diagram of recombinant plasmid pEP-BGH-GAstV ORF2
    Figure Legend Snippet: Schematic diagram of recombinant plasmid pEP-BGH-GAstV ORF2

    Techniques Used: Recombinant, Plasmid Preparation

    Recombinant pDEV-GAstV ORF2 construction. pDEV-EF1 (shown in the dotted box ( A and B )) were constructed and detailed in our prior publication . A . Homologous recombination was used to introduce a mini-F BAC vector (pHA2) that allows for large circular DNA to be maintained in E. coli into the intergenic region between UL15B and UL18 in a DEV vaccine strain. B . Two-step Red-mediated recombination ( en passant ) was used to substitute the P CMV promoter controlling GFP expression in pDEV-vac for the pEF1 gene. C - D . The recombinant BAC clone pDEV-kan.GAstV ORF2 was constructed by first inserting P CMV -GAstV ORF2-BGH-pA-Kan expression cassette into the US7 and US8 intergenic spacer in pDEV-EF1( C ) and pDEV-kan.GAstV ORF2 ( D ) were obtained by deleting Kan gene from pDEV-kan.GAstV ORF2 by 2nd homologous recombinant
    Figure Legend Snippet: Recombinant pDEV-GAstV ORF2 construction. pDEV-EF1 (shown in the dotted box ( A and B )) were constructed and detailed in our prior publication . A . Homologous recombination was used to introduce a mini-F BAC vector (pHA2) that allows for large circular DNA to be maintained in E. coli into the intergenic region between UL15B and UL18 in a DEV vaccine strain. B . Two-step Red-mediated recombination ( en passant ) was used to substitute the P CMV promoter controlling GFP expression in pDEV-vac for the pEF1 gene. C - D . The recombinant BAC clone pDEV-kan.GAstV ORF2 was constructed by first inserting P CMV -GAstV ORF2-BGH-pA-Kan expression cassette into the US7 and US8 intergenic spacer in pDEV-EF1( C ) and pDEV-kan.GAstV ORF2 ( D ) were obtained by deleting Kan gene from pDEV-kan.GAstV ORF2 by 2nd homologous recombinant

    Techniques Used: Recombinant, Construct, Homologous Recombination, Introduce, Plasmid Preparation, Expressing

    Bam HI digestion and PCR-based identification of recombinant BAC clones. a . Bam HI digestion-based analysis of pDEV-GAstV ORF2 mutants. 1: pDEV-EF1; 2: pDEV-Kan.GAstV ORF2; 3: pDEV-GAstV ORF2 mutants. M: 15,000 bp marker; 1: rDEV-EF1; 2: pDEV-Kan. GAstV ORF2; 3: pDEV-GAstV ORF2. b . PCR-based recombinant mutated BAC clone identification. 1: pDEV-EF1 (641 bp); 2: pDEV-Kan.GAstV ORF2 (4234 bp); 3 pDEV-GAstV ORF2 (3818 bp); M: 250 bp marker (4500, 3000, 2250, 1500, 1000, 750, 500, 250)
    Figure Legend Snippet: Bam HI digestion and PCR-based identification of recombinant BAC clones. a . Bam HI digestion-based analysis of pDEV-GAstV ORF2 mutants. 1: pDEV-EF1; 2: pDEV-Kan.GAstV ORF2; 3: pDEV-GAstV ORF2 mutants. M: 15,000 bp marker; 1: rDEV-EF1; 2: pDEV-Kan. GAstV ORF2; 3: pDEV-GAstV ORF2. b . PCR-based recombinant mutated BAC clone identification. 1: pDEV-EF1 (641 bp); 2: pDEV-Kan.GAstV ORF2 (4234 bp); 3 pDEV-GAstV ORF2 (3818 bp); M: 250 bp marker (4500, 3000, 2250, 1500, 1000, 750, 500, 250)

    Techniques Used: Recombinant, Clone Assay, Marker

    Rescued of recombinant virus. Fluorescence plaque of the rescued rDEV-GAstV ORF2 (100×)
    Figure Legend Snippet: Rescued of recombinant virus. Fluorescence plaque of the rescued rDEV-GAstV ORF2 (100×)

    Techniques Used: Recombinant, Virus, Fluorescence

    Plaque size of rDEV-GAstV ORF2 and control virus rDEV-EF1 on CEFs. Image J was used to compute means and standard deviations when measuring the sizes of 100 plaques, setting the mean plaque size for rDEV-EF1 to 100%. Error bars represent the standard deviation. The P value was calculated by the independent sample t -test, and a P value less than 0.05 was regarded as significant
    Figure Legend Snippet: Plaque size of rDEV-GAstV ORF2 and control virus rDEV-EF1 on CEFs. Image J was used to compute means and standard deviations when measuring the sizes of 100 plaques, setting the mean plaque size for rDEV-EF1 to 100%. Error bars represent the standard deviation. The P value was calculated by the independent sample t -test, and a P value less than 0.05 was regarded as significant

    Techniques Used: Control, Virus, Standard Deviation

    Multi-step growth curves of rDEV-GAstV ORF2 amd rDEV-EF1 on CEFs. Comparison of the in vitro growth of viruses reconstructed with parental DEV. The virus titers of infectedcells (A) and supernatants (B) were determined at different times (0, 12, 24, 36, 48, 60, and 72 h) after inoculation of approximately 0.02 MOI of cell-free viruses of rDEV-GAstV ORF2 and rDEV-EF1. The multi-step growth curves were computed from three independent experiments
    Figure Legend Snippet: Multi-step growth curves of rDEV-GAstV ORF2 amd rDEV-EF1 on CEFs. Comparison of the in vitro growth of viruses reconstructed with parental DEV. The virus titers of infectedcells (A) and supernatants (B) were determined at different times (0, 12, 24, 36, 48, 60, and 72 h) after inoculation of approximately 0.02 MOI of cell-free viruses of rDEV-GAstV ORF2 and rDEV-EF1. The multi-step growth curves were computed from three independent experiments

    Techniques Used: Comparison, In Vitro, Virus

    Analyses of GAstV Cap protein expression in virus-infected CEFs and supernatant samples by Western blot analysis. Detection of GAstV ORF2 protein ( A ) and inner protein DEV US3 ( C ) by Western blot analysis. Corresonding picture of SDS-PAGE ( C ). M: Prestained Protein Ladder (10-180 kDa); 1: rDEV-EF1-infected culture supernatants; 2: rDEV-GAstV ORF2-infected culture supernatants; 3: rDEV-EF1-infected CEFs; 4: rDEV-GAstV ORF2-infected CEFs
    Figure Legend Snippet: Analyses of GAstV Cap protein expression in virus-infected CEFs and supernatant samples by Western blot analysis. Detection of GAstV ORF2 protein ( A ) and inner protein DEV US3 ( C ) by Western blot analysis. Corresonding picture of SDS-PAGE ( C ). M: Prestained Protein Ladder (10-180 kDa); 1: rDEV-EF1-infected culture supernatants; 2: rDEV-GAstV ORF2-infected culture supernatants; 3: rDEV-EF1-infected CEFs; 4: rDEV-GAstV ORF2-infected CEFs

    Techniques Used: Expressing, Virus, Infection, Western Blot, SDS Page

    Analysis of GAstV Cap protein expression and localizaiton in CEFs by IFA. rDEV-GAstV ORF2 or control virus rDEV-EF1-infected CEFs were fixed and taken to IFA performed with anti-GAstV Cap mAb as primary antibody and Cy3-labelled goat anti-mouse IgG as secondary antibody. And then staining with DAPI solution. Red florescence represent Cap protien
    Figure Legend Snippet: Analysis of GAstV Cap protein expression and localizaiton in CEFs by IFA. rDEV-GAstV ORF2 or control virus rDEV-EF1-infected CEFs were fixed and taken to IFA performed with anti-GAstV Cap mAb as primary antibody and Cy3-labelled goat anti-mouse IgG as secondary antibody. And then staining with DAPI solution. Red florescence represent Cap protien

    Techniques Used: Expressing, Control, Virus, Infection, Staining

    Immunogold labeling electron microscopy analysis of Cap protein in rDEV-GAstV ORF2-infected CEFs. Nucleoprotein (N) immunogold labeling was achieved with an antibody against the Cap protein. GAstV Cap proteins are denoted by arrows pointing to colloidal gold spots. Scale bar: 200 nm; VLPs are marked with asterisks; Scale bar: 500 nm
    Figure Legend Snippet: Immunogold labeling electron microscopy analysis of Cap protein in rDEV-GAstV ORF2-infected CEFs. Nucleoprotein (N) immunogold labeling was achieved with an antibody against the Cap protein. GAstV Cap proteins are denoted by arrows pointing to colloidal gold spots. Scale bar: 200 nm; VLPs are marked with asterisks; Scale bar: 500 nm

    Techniques Used: Labeling, Electron Microscopy, Infection



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    gastv orf2  (GenScript corporation)
    90
    GenScript corporation gastv orf2
    Schematic diagram of recombinant plasmid <t>pEP-BGH-GAstV</t> <t>ORF2</t>
    Gastv Orf2, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/gastv orf2/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    gastv orf2 - by Bioz Stars, 2026-06
    90/100 stars
    		  Buy from Supplier

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    Schematic diagram of recombinant plasmid pEP-BGH-GAstV ORF2

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Schematic diagram of recombinant plasmid pEP-BGH-GAstV ORF2

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Recombinant, Plasmid Preparation

    Recombinant pDEV-GAstV ORF2 construction. pDEV-EF1 (shown in the dotted box ( A and B )) were constructed and detailed in our prior publication . A . Homologous recombination was used to introduce a mini-F BAC vector (pHA2) that allows for large circular DNA to be maintained in E. coli into the intergenic region between UL15B and UL18 in a DEV vaccine strain. B . Two-step Red-mediated recombination ( en passant ) was used to substitute the P CMV promoter controlling GFP expression in pDEV-vac for the pEF1 gene. C - D . The recombinant BAC clone pDEV-kan.GAstV ORF2 was constructed by first inserting P CMV -GAstV ORF2-BGH-pA-Kan expression cassette into the US7 and US8 intergenic spacer in pDEV-EF1( C ) and pDEV-kan.GAstV ORF2 ( D ) were obtained by deleting Kan gene from pDEV-kan.GAstV ORF2 by 2nd homologous recombinant

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Recombinant pDEV-GAstV ORF2 construction. pDEV-EF1 (shown in the dotted box ( A and B )) were constructed and detailed in our prior publication . A . Homologous recombination was used to introduce a mini-F BAC vector (pHA2) that allows for large circular DNA to be maintained in E. coli into the intergenic region between UL15B and UL18 in a DEV vaccine strain. B . Two-step Red-mediated recombination ( en passant ) was used to substitute the P CMV promoter controlling GFP expression in pDEV-vac for the pEF1 gene. C - D . The recombinant BAC clone pDEV-kan.GAstV ORF2 was constructed by first inserting P CMV -GAstV ORF2-BGH-pA-Kan expression cassette into the US7 and US8 intergenic spacer in pDEV-EF1( C ) and pDEV-kan.GAstV ORF2 ( D ) were obtained by deleting Kan gene from pDEV-kan.GAstV ORF2 by 2nd homologous recombinant

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Recombinant, Construct, Homologous Recombination, Introduce, Plasmid Preparation, Expressing

    Bam HI digestion and PCR-based identification of recombinant BAC clones. a . Bam HI digestion-based analysis of pDEV-GAstV ORF2 mutants. 1: pDEV-EF1; 2: pDEV-Kan.GAstV ORF2; 3: pDEV-GAstV ORF2 mutants. M: 15,000 bp marker; 1: rDEV-EF1; 2: pDEV-Kan. GAstV ORF2; 3: pDEV-GAstV ORF2. b . PCR-based recombinant mutated BAC clone identification. 1: pDEV-EF1 (641 bp); 2: pDEV-Kan.GAstV ORF2 (4234 bp); 3 pDEV-GAstV ORF2 (3818 bp); M: 250 bp marker (4500, 3000, 2250, 1500, 1000, 750, 500, 250)

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Bam HI digestion and PCR-based identification of recombinant BAC clones. a . Bam HI digestion-based analysis of pDEV-GAstV ORF2 mutants. 1: pDEV-EF1; 2: pDEV-Kan.GAstV ORF2; 3: pDEV-GAstV ORF2 mutants. M: 15,000 bp marker; 1: rDEV-EF1; 2: pDEV-Kan. GAstV ORF2; 3: pDEV-GAstV ORF2. b . PCR-based recombinant mutated BAC clone identification. 1: pDEV-EF1 (641 bp); 2: pDEV-Kan.GAstV ORF2 (4234 bp); 3 pDEV-GAstV ORF2 (3818 bp); M: 250 bp marker (4500, 3000, 2250, 1500, 1000, 750, 500, 250)

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Recombinant, Clone Assay, Marker

    Rescued of recombinant virus. Fluorescence plaque of the rescued rDEV-GAstV ORF2 (100×)

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Rescued of recombinant virus. Fluorescence plaque of the rescued rDEV-GAstV ORF2 (100×)

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Recombinant, Virus, Fluorescence

    Plaque size of rDEV-GAstV ORF2 and control virus rDEV-EF1 on CEFs. Image J was used to compute means and standard deviations when measuring the sizes of 100 plaques, setting the mean plaque size for rDEV-EF1 to 100%. Error bars represent the standard deviation. The P value was calculated by the independent sample t -test, and a P value less than 0.05 was regarded as significant

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Plaque size of rDEV-GAstV ORF2 and control virus rDEV-EF1 on CEFs. Image J was used to compute means and standard deviations when measuring the sizes of 100 plaques, setting the mean plaque size for rDEV-EF1 to 100%. Error bars represent the standard deviation. The P value was calculated by the independent sample t -test, and a P value less than 0.05 was regarded as significant

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Control, Virus, Standard Deviation

    Multi-step growth curves of rDEV-GAstV ORF2 amd rDEV-EF1 on CEFs. Comparison of the in vitro growth of viruses reconstructed with parental DEV. The virus titers of infectedcells (A) and supernatants (B) were determined at different times (0, 12, 24, 36, 48, 60, and 72 h) after inoculation of approximately 0.02 MOI of cell-free viruses of rDEV-GAstV ORF2 and rDEV-EF1. The multi-step growth curves were computed from three independent experiments

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Multi-step growth curves of rDEV-GAstV ORF2 amd rDEV-EF1 on CEFs. Comparison of the in vitro growth of viruses reconstructed with parental DEV. The virus titers of infectedcells (A) and supernatants (B) were determined at different times (0, 12, 24, 36, 48, 60, and 72 h) after inoculation of approximately 0.02 MOI of cell-free viruses of rDEV-GAstV ORF2 and rDEV-EF1. The multi-step growth curves were computed from three independent experiments

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Comparison, In Vitro, Virus

    Analyses of GAstV Cap protein expression in virus-infected CEFs and supernatant samples by Western blot analysis. Detection of GAstV ORF2 protein ( A ) and inner protein DEV US3 ( C ) by Western blot analysis. Corresonding picture of SDS-PAGE ( C ). M: Prestained Protein Ladder (10-180 kDa); 1: rDEV-EF1-infected culture supernatants; 2: rDEV-GAstV ORF2-infected culture supernatants; 3: rDEV-EF1-infected CEFs; 4: rDEV-GAstV ORF2-infected CEFs

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Analyses of GAstV Cap protein expression in virus-infected CEFs and supernatant samples by Western blot analysis. Detection of GAstV ORF2 protein ( A ) and inner protein DEV US3 ( C ) by Western blot analysis. Corresonding picture of SDS-PAGE ( C ). M: Prestained Protein Ladder (10-180 kDa); 1: rDEV-EF1-infected culture supernatants; 2: rDEV-GAstV ORF2-infected culture supernatants; 3: rDEV-EF1-infected CEFs; 4: rDEV-GAstV ORF2-infected CEFs

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Expressing, Virus, Infection, Western Blot, SDS Page

    Analysis of GAstV Cap protein expression and localizaiton in CEFs by IFA. rDEV-GAstV ORF2 or control virus rDEV-EF1-infected CEFs were fixed and taken to IFA performed with anti-GAstV Cap mAb as primary antibody and Cy3-labelled goat anti-mouse IgG as secondary antibody. And then staining with DAPI solution. Red florescence represent Cap protien

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Analysis of GAstV Cap protein expression and localizaiton in CEFs by IFA. rDEV-GAstV ORF2 or control virus rDEV-EF1-infected CEFs were fixed and taken to IFA performed with anti-GAstV Cap mAb as primary antibody and Cy3-labelled goat anti-mouse IgG as secondary antibody. And then staining with DAPI solution. Red florescence represent Cap protien

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Expressing, Control, Virus, Infection, Staining

    Immunogold labeling electron microscopy analysis of Cap protein in rDEV-GAstV ORF2-infected CEFs. Nucleoprotein (N) immunogold labeling was achieved with an antibody against the Cap protein. GAstV Cap proteins are denoted by arrows pointing to colloidal gold spots. Scale bar: 200 nm; VLPs are marked with asterisks; Scale bar: 500 nm

    Journal: BMC Veterinary Research

    Article Title: In vitro expression of the goose astrovirus Cap protein delivered with a duck enteritis virus vector

    doi: 10.1186/s12917-025-04654-7

    Figure Lengend Snippet: Immunogold labeling electron microscopy analysis of Cap protein in rDEV-GAstV ORF2-infected CEFs. Nucleoprotein (N) immunogold labeling was achieved with an antibody against the Cap protein. GAstV Cap proteins are denoted by arrows pointing to colloidal gold spots. Scale bar: 200 nm; VLPs are marked with asterisks; Scale bar: 500 nm

    Article Snippet: GAstV ORF2 containing separate Not I and Kpn I sites introduced into the 5’and 3’ termini was optimized according to the duck codon preferences, after which it was synthesized by GeneScript (Nanjing, China) with reference to the GAstV gene sequence (GenBank MH052598).

    Techniques: Labeling, Electron Microscopy, Infection